8TCL
Crystal Structure of modified HIV reverse transcriptase p51 domain (FPC2) with picrate bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-09-19 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.514 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 77.579, 99.624, 104.161 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.620 - 1.950 |
R-factor | 0.1998 |
Rwork | 0.198 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.160 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmeas | 0.054 | 0.473 |
Rpim | 0.024 | 9.268 |
Number of reflections | 28616 | 1214 |
<I/σ(I)> | 10.7 | |
Completeness [%] | 96.1 | 84.1 |
Redundancy | 4.2 | |
CC(1/2) | 0.862 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | protein: 25mg/ml 10mM Tris pH 7.4, 40mM NaCl, 1mM TCEP, 0.25mM azide, 10mM picric acid ML: 20%PEG3350, 0.2M trisodium citrate |