8TCK
Apo crystal Structure of modified HIV reverse transcriptase p51 domain (FPC1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-11-30 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.514 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.735, 63.952, 128.296 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.259 - 2.200 |
R-factor | 0.2091 |
Rwork | 0.207 |
R-free | 0.23960 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.730 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmeas | 0.073 | 0.448 |
Rpim | 0.021 | 0.165 |
Number of reflections | 22917 | 990 |
<I/σ(I)> | 9.5 | |
Completeness [%] | 98.8 | 86.1 |
Redundancy | 11.8 | |
CC(1/2) | 0.895 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | Protein: 25mg/ml 50mM NaCl, 10mM Tris pH 8.0, 2mM DTT ML: 11% PEG 6000, 0.1M Tris pH 8.5 |