8TCK

Apo crystal Structure of modified HIV reverse transcriptase p51 domain (FPC1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-30
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.514
Spacegroup name	P 21 21 21
Unit cell lengths	53.735, 63.952, 128.296
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.259 - 2.200
R-factor	0.2091
Rwork	0.207
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.730
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmeas	0.073	0.448
Rpim	0.021	0.165
Number of reflections	22917	990
<I/σ(I)>	9.5
Completeness [%]	98.8	86.1
Redundancy	11.8
CC(1/2)		0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	Protein: 25mg/ml 50mM NaCl, 10mM Tris pH 8.0, 2mM DTT ML: 11% PEG 6000, 0.1M Tris pH 8.5