8TCJ
Apo crystal Structure of modified HIV reverse transcriptase p51 domain (FPC2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-04 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.514 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 105.080, 105.080, 54.531 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.776 - 1.848 |
R-factor | 0.2177 |
Rwork | 0.216 |
R-free | 0.25230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.257 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.848 | 1.850 |
Rmeas | 0.039 | |
Number of reflections | 29994 | 1497 |
<I/σ(I)> | 24.3 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.2 | 2.2 |
CC(1/2) | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Protein: 25mg/ml FPC2, 50mM NaCl, 10mM Tris pH 7.0, 1mM TCEP, 5%DMSO, 0.25mM azide, 5mM fisetin Res: 25% PEG1500, 0.1M TBG pH 7.0 |