8TBV

Human Class I MHC HLA-A2 in complex with sorting nexin 24 (127-135) neoantigen KLSHQLVLL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	62.226, 86.726, 79.268
Unit cell angles	90.00, 90.07, 90.00

Refinement procedure

Resolution	62.230 - 2.640
R-factor	0.2337
Rwork	0.229
R-free	0.27440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.421
Data reduction software	DIALS (2.2.10)
Data scaling software	DIALS (2.2.10)
Phasing software	PHENIX (1.20rc3_4406)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.230	2.734
High resolution limit [Å]	2.640	2.640
Number of reflections	23797	2209
<I/σ(I)>	4.32
Completeness [%]	92.0	88.75
Redundancy	6.6
CC(1/2)	0.944	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	Purified protein complexes were concentrated to 7.4 mg/mL before crystallization. The crystals were grown in 12.5% PEG 4000 and 0.1 M MES (pH 6.5) via hanging drop vapor diffusion. Crystals were harvested and cryoprotected in 8% glycerol/92% mother liquor and immediately stored in liquid nitrogen