8TBM

Tricomplex of RMC-7977, KRAS G12V, and CypA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	65.560, 84.090, 127.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.580 - 1.570
R-factor	0.1797
Rwork	0.178
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.859
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.580	1.626
High resolution limit [Å]	1.570	1.570
Rmerge	0.106	2.779
Rmeas	0.115	2.967
Rpim	0.044	1.136
Number of reflections	96907	9506
<I/σ(I)>	10.67	0.93
Completeness [%]	98.2	97.67
Redundancy	6.6	6.9
CC(1/2)	0.998	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	24% PEG6000, 0.1 M imidazole, pH 7