8TBK

Tricomplex of RMC-7977, KRAS G12C, and CypA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95375
Spacegroup name	P 21 21 21
Unit cell lengths	65.470, 83.570, 126.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.920 - 1.260
R-factor	0.1481
Rwork	0.146
R-free	0.18390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.868
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.920	1.305
High resolution limit [Å]	1.260	1.260
Rmerge	0.075	1.949
Rmeas	0.080	2.092
Rpim	0.029	0.752
Number of reflections	183467	17699
<I/σ(I)>	11.03
Completeness [%]	98.3	95.69
Redundancy	7.6	7.5
CC(1/2)	0.999	0.367

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	27% PEG4000, 0.1 M imidazole, pH 7.0