8TBH

Tricomplex of RMC-7977, KRAS G12R, and CypA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95375
Spacegroup name	P 21 21 21
Unit cell lengths	66.418, 85.673, 127.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.770 - 1.500
R-factor	0.1974
Rwork	0.196
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.809
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.770	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.105	2.692
Rmeas	0.113	2.888
Rpim	0.041	1.035
Number of reflections	116906	11564
<I/σ(I)>	7.51	0.55
Completeness [%]	99.8	99.25
Redundancy	7.3	7.5
CC(1/2)	0.998	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	17% PEG4000, 0.1 M imidazole, pH 8.0