8TAT
CRYSTAL STRUCTURE OF R9A SPIN LABELED T4 LYSOZYME MUTANT K65R9A/R76R9A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-07-13 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.390, 60.390, 95.430 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.300 - 1.600 |
R-factor | 0.1988 |
Rwork | 0.198 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.874 |
Data reduction software | XDS (20210323) |
Data scaling software | XSCALE (20210323) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 52.300 | 52.300 | 1.640 |
High resolution limit [Å] | 1.600 | 7.150 | 1.600 |
Rmerge | 0.082 | 0.047 | 0.962 |
Rmeas | 0.086 | 0.049 | 1.011 |
Number of reflections | 50346 | 578 | 3627 |
<I/σ(I)> | 16.04 | ||
Completeness [%] | 97.8 | ||
Redundancy | 10.7 | ||
CC(1/2) | 0.999 | 0.999 | 0.837 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 2.35M DIBASIC POTASSIUM PHOSPHATE AND MONOBASIC SODIUM PHOSPHATE, 0.15M SODIUM CHLORIDE, 100mM 1,6-HEXANEDIOL, 3% 2-PROPANOL, PH 7.4, VAPOR DIFFUSION, HANGING DROP |