8TAC
Designed DNA binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-25 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.352, 103.996, 32.825 |
| Unit cell angles | 90.00, 99.47, 90.00 |
Refinement procedure
| Resolution | 52.000 - 2.340 |
| R-factor | 0.248 |
| Rwork | 0.244 |
| R-free | 0.27910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.579 |
| Data reduction software | DIALS (3.8) |
| Data scaling software | DIALS (3.8) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.000 | 2.424 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.183 | 0.300 |
| Rmeas | 0.200 | 0.327 |
| Rpim | 0.079 | 0.130 |
| Number of reflections | 7888 | 772 |
| <I/σ(I)> | 5.38 | 1.05 |
| Completeness [%] | 99.6 | 99.87 |
| Redundancy | 6.2 | 6.2 |
| CC(1/2) | 0.979 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 300 | 0.2 M Ammonium acetate, 0.1 M Sodium acetate pH 4.6, 28% PEG 4000 |
