8T9Y
Structure of VHH-Fab complex with engineered Elbow FNQIKG and Crystal Kappa regions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-14 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.565, 73.565, 207.235 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.520 |
R-factor | 0.21802 |
Rwork | 0.214 |
R-free | 0.28482 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.053 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 103.620 | 2.620 |
High resolution limit [Å] | 2.520 | 2.520 |
Rpim | 0.030 | 0.510 |
Number of reflections | 20161 | 2220 |
<I/σ(I)> | 19.2 | 1.1 |
Completeness [%] | 100.0 | 99.7 |
Redundancy | 25.9 | |
CC(1/2) | 0.990 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 0.1 M Citric acid, 25% PEG 3350 |