8T8A
Structure of arginine oxidase from Pseudomonas sp. TRU 7192
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 168.274, 200.219, 168.542 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.040 - 3.400 |
| R-factor | 0.21698 |
| Rwork | 0.212 |
| R-free | 0.31298 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.421 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.230 | 3.470 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.346 | 0.977 |
| Rpim | 0.144 | 0.401 |
| Number of reflections | 39436 | 4532 |
| <I/σ(I)> | 4.6 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG3350, 0.2M Potassium citrate |
