8T6N
Crystal structure of T33-27.1: Deep-learning sequence design of co-assembling tetrahedron protein nanoparticles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-12-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99994 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 176.515, 176.515, 176.515 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.960 - 3.630 |
| R-factor | 0.2186 |
| Rwork | 0.214 |
| R-free | 0.25850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.432 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4761) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.720 |
| High resolution limit [Å] | 3.630 | 3.630 |
| Rmerge | 0.229 | 0.063 |
| Number of reflections | 20978 | 4972 |
| <I/σ(I)> | 22.69 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.5 | 21.4 |
| CC(1/2) | 0.998 | 0.961 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.12 M ethylene glycol, 0.1 M Tris-Bicine, pH 8.5, 30% v/v glycerol + PEG4000 |
