8T6I
Structure of VHH-Fab complex with engineered Crystal Kappa region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-01 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.481, 74.189, 218.423 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.368 - 2.550 |
| R-factor | 0.1972 |
| Rwork | 0.194 |
| R-free | 0.25450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.101 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.190 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rpim | 0.030 | 0.800 |
| Number of reflections | 29702 | 3539 |
| <I/σ(I)> | 18.5 | 1 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.6 | 5.8 |
| CC(1/2) | 0.990 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Potassium citrate tribasic monohydrate, 20% PEG3350 |
