8T67

Influenza PA-N Endonuclease I38T Mutant (amino acids 52-74 truncation)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00
Spacegroup name	P 62 2 2
Unit cell lengths	74.589, 74.589, 119.534
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.600 - 2.080
R-factor	0.2103
Rwork	0.206
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.823
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.620	2.130
High resolution limit [Å]	2.080	2.080
Number of reflections	22347	1068
<I/σ(I)>	18
Completeness [%]	100.0
Redundancy	20.2
CC(1/2)	0.999	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		306	32% PEG (MW 4000 g/mol), 100 mM Tris (pH 8.35), and 220 mM sodium acetate