8T66
cA6 bound Cam1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.352, 75.813, 125.195 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.600 - 1.850 |
| R-factor | 0.1969 |
| Rwork | 0.196 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.947 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 125.200 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.214 | 1.010 |
| Rpim | 0.390 | |
| Number of reflections | 28724 | 1488 |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 99.1 | |
| Redundancy | 8.7 | |
| CC(1/2) | 0.998 | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.05 M Magnesium Chloride hexahydrate, 0.1 M HEPES, pH 7.5, 30% (w/v) PEGMME 550 |
