8T65
cA4 bound Cam1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.987, 64.861, 50.129 |
| Unit cell angles | 90.00, 113.28, 90.00 |
Refinement procedure
| Resolution | 46.050 - 2.090 |
| R-factor | 0.1741 |
| Rwork | 0.172 |
| R-free | 0.22080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.904 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.17.1_3660)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.860 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.069 | 0.301 |
| Number of reflections | 16011 | 918 |
| <I/σ(I)> | 14.6 | |
| Completeness [%] | 97.3 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.998 | 0.938 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M ammonium sulfate, 0.1 M phosphate citrate, pH 4.2, 20% (w/v) PEG300, 10% Glycerol |
