8T64

Apo Cam1(42-206)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	43.783, 120.551, 51.544
Unit cell angles	90.00, 115.34, 90.00

Refinement procedure

Resolution	46.590 - 2.250
R-factor	0.2071
Rwork	0.205
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.281
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.17.1_3660))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.083
Number of reflections	96801	22371
<I/σ(I)>	24.3
Completeness [%]	97.8
Redundancy	4.3
CC(1/2)	0.996	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M sodium chloride, 0.1 M sodium cacodylate trihydrate, pH 6.6, 25% PEG3350 (w/v)