8T58

Structure of VHH-Fab complex with engineered FNQIKG elbow region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	65.771, 119.826, 82.244
Unit cell angles	90.00, 93.35, 90.00

Refinement procedure

Resolution	67.730 - 2.230
R-factor	0.18451
Rwork	0.182
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.035
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.830	2.290
High resolution limit [Å]	2.230	2.230
Rpim	0.030	0.240
Number of reflections	61587	4313
<I/σ(I)>	16.1	2.7
Completeness [%]	99.1
Redundancy	6.9
CC(1/2)	0.990	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	292	4% Tacsimate, 12% PEG 3350