8T4T
Crystal structure of LC3A in complex with the LIR of TP53INP2/DOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.004, 47.740, 72.902 |
Unit cell angles | 90.00, 95.49, 90.00 |
Refinement procedure
Resolution | 39.883 - 2.359 |
R-factor | 0.2101 |
Rwork | 0.203 |
R-free | 0.27150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.231 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.883 | 2.444 |
High resolution limit [Å] | 2.359 | 2.359 |
Number of reflections | 9618 | 508 |
<I/σ(I)> | 7.91 | |
Completeness [%] | 89.7 | |
Redundancy | 3 | |
CC(1/2) | 0.995 | 0.123 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12.5% PEG3350, 0.2M NH4-citrate, 5% isopropanol, 0.1M MES pH6.5 |