8T4T

Crystal structure of LC3A in complex with the LIR of TP53INP2/DOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	37.004, 47.740, 72.902
Unit cell angles	90.00, 95.49, 90.00

Refinement procedure

Resolution	39.883 - 2.359
R-factor	0.2101
Rwork	0.203
R-free	0.27150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.231
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.883	2.444
High resolution limit [Å]	2.359	2.359
Number of reflections	9618	508
<I/σ(I)>	7.91
Completeness [%]	89.7
Redundancy	3
CC(1/2)	0.995	0.123

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	12.5% PEG3350, 0.2M NH4-citrate, 5% isopropanol, 0.1M MES pH6.5