8T3P
Crystal structure of MonC1 (a flavin-dependent monooxygenase)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.153, 52.353, 141.561 |
| Unit cell angles | 90.00, 93.60, 90.00 |
Refinement procedure
| Resolution | 39.720 - 1.900 |
| R-factor | 0.1836 |
| Rwork | 0.181 |
| R-free | 0.22230 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.987 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.20.1_4487) |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 28.5% w/v PEG MME 2000, 0.1M Tris (pH 6.5), 0.1M MOPS (pH 6.8), 4% Glycerol |
