8T32

Crystal structure of K48 acetylated GABARAP in complex with the LIR of TP53INP2/DOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96870
Spacegroup name	P 42 21 2
Unit cell lengths	44.179, 44.179, 128.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.800 - 2.051
R-factor	0.2566
Rwork	0.255
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.463
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.800	2.124
High resolution limit [Å]	2.051	2.051
Number of reflections	8486	648
<I/σ(I)>	16.91
Completeness [%]	97.3
Redundancy	20
CC(1/2)	0.999	0.408

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	28 % (w/v) PEG 2000 MME, 0.1 M Bis-Tris: HCl, pH 6.5