8T2N

Crystal structure of GABARAP in complex with the LIR of NSs3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96860
Spacegroup name	C 1 2 1
Unit cell lengths	138.725, 31.485, 68.917
Unit cell angles	90.00, 115.81, 90.00

Refinement procedure

Resolution	36.740 - 1.880
R-factor	0.2248
Rwork	0.221
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.557
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.740	1.947
High resolution limit [Å]	1.880	1.880
Number of reflections	20163	833
<I/σ(I)>	14.13
Completeness [%]	88.4
Redundancy	5.5
CC(1/2)	1.000	0.105

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Potassium acetate, 20% PEG 3350