8T2B

Crystal structure of SCV PTE G18C mutant RNA in complex with Fab BL3-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-15
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	I 2 2 2
Unit cell lengths	77.719, 81.570, 324.504
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	162.250 - 3.180
R-factor	0.202
Rwork	0.198
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.311
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	162.250	3.400
High resolution limit [Å]	3.180	3.180
Rmerge	0.055	1.732
Rmeas	0.060	1.882
Rpim	0.024	0.727
Number of reflections	17792	3175
<I/σ(I)>	14.6	0.9
Completeness [%]	99.6	99.6
Redundancy	6.5	6.6
CC(1/2)	0.999	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	298	0.2 M lithium sulfate monohydrate, 0.1 M tris pH 8.6, 20% w/v PEG 3350