8T1V

Crystal structure of orphan G protein-coupled receptor 6 with bound inverse agonist 3h

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	123
Detector technology	PIXEL
Collection date	2019-08-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	63.136, 63.136, 249.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.130 - 2.600
R-factor	0.2327
Rwork	0.230
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.442
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.147	0.693
Number of reflections	14570	467
<I/σ(I)>	9.1	1.4
Completeness [%]	78.2
Redundancy	6.6
CC(1/2)	0.986	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	NaCH3COO, PEG 400, NaCl, PPG P40