8T1S

Structure of the alpha-N-methyltransferase (SonM) and RiPP precursor (SonA with QSY deletion) heteromeric complex (bound to SAH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-03
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	52.390, 108.730, 59.140
Unit cell angles	90.00, 93.66, 90.00

Refinement procedure

Resolution	52.280 - 2.000
R-factor	0.2045
Rwork	0.203
R-free	0.23814
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.553
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.280	2.100
High resolution limit [Å]	2.000	2.000
Rmeas	0.111	0.582
Number of reflections	44465	6044
<I/σ(I)>	10.89
Completeness [%]	99.7
Redundancy	5.41
CC(1/2)	0.997	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	240 mM sodium malonate pH 5-5.5, 8-12% PEG 3350 at a 1-1 or 2-1 ratio of protein to mother liquor. Protein concentration is 20mg/mL.