8T0P

Structure of Cse4 bound to Ame1 and Okp1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	CCD
Collection date	2022-09-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 42 21 2
Unit cell lengths	154.305, 154.305, 37.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.010 - 1.730
R-factor	0.2082
Rwork	0.207
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.554
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.110	1.760
High resolution limit [Å]	1.730	1.730
Rmerge	0.125
Rmeas	0.128
Rpim	0.025
Number of reflections	47590	2301
<I/σ(I)>	16.93	0.95
Completeness [%]	100.0	97.75
Redundancy	14.2
CC(1/2)	1.000	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	19% PEG 8000, 0.55 M Lithium sulfate