8T0O
Fab from mAb RB2AT_87
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 113 |
| Detector technology | PIXEL |
| Collection date | 2020-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 111.358, 111.358, 79.156 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.450 - 2.300 |
| R-factor | 0.2186 |
| Rwork | 0.215 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.945 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.450 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 44577 | 2190 |
| <I/σ(I)> | 9.11 | |
| Completeness [%] | 91.6 | |
| Redundancy | 30 | |
| CC(1/2) | 0.999 | 0.339 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris at pH 8.0 and 2.0 M (NH4)2SO4 |
