8SZQ

Cat DHX9 bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95364
Spacegroup name	P 43 21 2
Unit cell lengths	86.010, 86.010, 348.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.170 - 2.711
Rwork	0.226
R-free	0.27350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.708
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.170	2.830
High resolution limit [Å]	2.710	2.710
Rmerge	0.127	1.519
Rmeas	0.129	1.549
Rpim	0.025	0.299
Total number of observations	959448	110071
Number of reflections	36693	4317
<I/σ(I)>	23.1	2.9
Completeness [%]	99.8
Redundancy	26.1	25.5
CC(1/2)	1.000	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.15 M Ammonium sulfate, 0.1 M TRIS pH 8, 19% (w/v) PEG 4000; 5 mM ADP and 50 mM magnesium chloride in protein solution