8SXL
RNA UU template binding to AMP monomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2021-03-14 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 43.838, 43.838, 82.845 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.990 - 1.900 |
| R-factor | 0.21921 |
| Rwork | 0.217 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8swg |
| RMSD bond length | 0.023 |
| RMSD bond angle | 3.021 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | 0.501 |
| Number of reflections | 7680 | 737 |
| <I/σ(I)> | 28.9 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 9.1 |
| CC(1/2) | 0.981 | 0.911 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 10% v/v MPD, 0.040 M sodium cacodylate trihydrate, pH 7.0, 0.012 M spermine tetrahydrochloride, 0.020 M magnesium chloride hexahydrate |
