8SWI

Crystal structure of legAS4 from Legionella pneumophila subsp. pneumophila with histone H3 (1-12)peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-06
Detector	RAYONIX MX-300
Wavelength(s)	1.5212
Spacegroup name	P 31 2 1
Unit cell lengths	70.290, 70.290, 200.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.980 - 3.000
R-factor	0.254
Rwork	0.251
R-free	0.31050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.370
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	12117	1201
<I/σ(I)>	12.89
Completeness [%]	99.9
Redundancy	9
CC(1/2)	0.996	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M Bis-Tris 6.5 and 38% PPG P400