8SWG
RNA duplex bound with GpppA dinucleotide ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2022-10-15 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 43.543, 43.543, 84.269 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.740 - 1.500 |
| R-factor | 0.22029 |
| Rwork | 0.218 |
| R-free | 0.26634 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hbx |
| RMSD bond length | 0.021 |
| RMSD bond angle | 3.254 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.084 | 0.564 |
| Rmeas | 0.089 | 0.603 |
| Rpim | 0.029 | 0.212 |
| Number of reflections | 15337 | 1506 |
| <I/σ(I)> | 21.2 | 3.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.2 | 8 |
| CC(1/2) | 0.966 | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M Bis-Tris, pH 6.5, 2 M ammonium sulfate |
