8SVZ

Structure of the Francisella response regulator KdpE receiver domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 62 2 2
Unit cell lengths	134.708, 134.708, 123.118
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.440 - 3.350
R-factor	0.2301
Rwork	0.228
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.615
Data reduction software	HKL-2000 (720)
Data scaling software	HKL-2000 (720)
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.400
High resolution limit [Å]	3.340	3.340
Rmeas	0.193	7.345
Rpim	0.057	2.345
Number of reflections	10007	487
<I/σ(I)>	24.825	0.326
Completeness [%]	100.0	100
Redundancy	12.1	9.2
CC(1/2)	0.961	0.120

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	1.5 M ammonium sulfate, 0.1 M Tris pH 8.5, 12% glycerol