8SVI

Ubiquitin variant i53:Mutant L67H with 53BP1 Tudor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95372
Spacegroup name	P 21 21 21
Unit cell lengths	40.380, 46.870, 89.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.210 - 1.150
Rwork	0.205
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.210	1.170
High resolution limit [Å]	1.150	1.150
Rmerge		0.986
Rpim	0.034
Number of reflections	59957	5255
<I/σ(I)>	10.1	1.79
Completeness [%]	98.0	86.9
Redundancy	7
CC(1/2)	0.997	0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	0.1 M MES pH 6.0, 0.2 M Trimethylamine N-oxide dehydrate, 11% w/v PEG MME 2000