8SVG

Ubiquitin variant i53 in complex with 53BP1 Tudor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95372
Spacegroup name	P 21 21 21
Unit cell lengths	40.370, 46.860, 90.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.300 - 1.210
Rwork	0.180
R-free	0.18920
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.330	1.230
High resolution limit [Å]	1.210	1.210
Rmeas	0.057
Rpim	0.021
Number of reflections	51451	4865
<I/σ(I)>	18.68
Completeness [%]	96.9	93.5
Redundancy	7.5	7.5
CC(1/2)	0.999	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	0.1 M MES pH 6.4, 0.2 M Trimethylamine N-oxide dehydrate, 2% w/v PEG2000