8SV7

The 1.53 Angstrom structure of human Tango2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	49.730, 49.960, 241.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.710 - 1.530
Rwork	0.168
R-free	0.19570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.796
Data reduction software	XDS (V. 220110)
Data scaling software	XDS (V. 220110)
Phasing software	PHENIX ((1.20.1_4487: ???))
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.710	1.570
High resolution limit [Å]	1.530	1.530
Rmerge	0.045	0.499
Rmeas	0.049	0.525
Rpim	0.130
Number of reflections	84855	4390
<I/σ(I)>	28.14	3.97
Completeness [%]	94.2	65.1
Redundancy	13.6
CC(1/2)	1.000	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	Equal parts of protein solution 25 mg/ml and a precipitate cocktail consisting of 1.0 M Ammonium sulfate, 0.1 M BIS-TRIS pH 5.5, 1% w/v Polyethylene glycol 3,350