8SV0

The crystal structure of the classical binding interface of Importin alpha 2 and nuclear localisation signal sequence in Psittacine siadenovirus core protein VII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	77.217, 88.728, 96.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.670 - 2.200
R-factor	0.2304
Rwork	0.229
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ukx
RMSD bond length	0.010
RMSD bond angle	1.415
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.670	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.163	1.791
Rpim	0.075	0.842
Number of reflections	34168	2903
<I/σ(I)>	4
Completeness [%]	99.7	99.5
Redundancy	5.3
CC(1/2)	0.992	0.319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	0.6 M sodium citrate, 0.1 M sodium HEPES, 1% dithiothreitol