8SSS

ZnFs 1-7 of CCCTC-binding factor (CTCF) Complexed with 23mer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	161.013, 41.147, 135.661
Unit cell angles	90.00, 105.18, 90.00

Refinement procedure

Resolution	43.180 - 2.300
R-factor	0.2005
Rwork	0.199
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.465
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.180	2.370
High resolution limit [Å]	2.290	2.290
Rmerge	0.090	0.989
Rpim	0.038	0.649
Number of reflections	36617	2757
<I/σ(I)>	17	1.1
Completeness [%]	94.0	74.4
Redundancy	6	2.5
CC(1/2)	1.000	0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	26.5% PEG3350, 0.26M DL-Malic acid 7.0