8SSQ
ZnFs 3-11 of CCCTC-binding factor (CTCF) Complexed with 35mer DNA 35-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 352.070, 67.768, 61.194 |
| Unit cell angles | 90.00, 92.59, 90.00 |
Refinement procedure
| Resolution | 41.860 - 3.120 |
| R-factor | 0.2412 |
| Rwork | 0.240 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.472 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.860 | 3.230 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Rpim | 0.076 | 0.703 |
| Number of reflections | 49980 | 5013 |
| <I/σ(I)> | 11 | 1.2 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 27.1 | 11.5 |
| CC(1/2) | 0.985 | 0.397 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M Sodium malonate pH 5.0, 15-22% w/v PEG 3,350 |
