8SSK
Citrobacter rodentium contact dependent growth inhibition (CDI) entry and toxin (CdiA-CT) domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.870, 113.982, 87.351 |
| Unit cell angles | 90.00, 92.39, 90.00 |
Refinement procedure
| Resolution | 39.260 - 1.900 |
| R-factor | 0.1688 |
| Rwork | 0.166 |
| R-free | 0.19370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.651 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.260 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.118 | 1.187 |
| Rmeas | 0.140 | 1.409 |
| Rpim | 0.074 | 0.748 |
| Number of reflections | 101283 | 4996 |
| <I/σ(I)> | 11.4 | 1.6 |
| Completeness [%] | 98.5 | 98.7 |
| Redundancy | 7 | 7 |
| CC(1/2) | 0.998 | 0.746 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 0.1 M sodium acetate, pH 4, 2 M ammonium sulfate |
