8SSI
Structure of Burkholderia pseudomallei deubiquitinase TssM in complex with ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.999903 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 104.517, 104.517, 193.801 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.760 - 2.500 |
R-factor | 0.2283 |
Rwork | 0.227 |
R-free | 0.25360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.590 |
Data reduction software | DIALS (3.10) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.760 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.045 | 0.393 |
Number of reflections | 38023 | 4205 |
<I/σ(I)> | 8.9 | 1.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 1.9 | 1.9 |
CC(1/2) | 0.996 | 0.360 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 1.4 M Sodium phosphate monobasic monohydrate/Potassium phosphate dibasic pH 5.6 |