8SS1
Structure of a bacterial death-like domain from Azospirillum sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-05-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.959, 65.367, 38.829 |
| Unit cell angles | 90.00, 111.06, 90.00 |
Refinement procedure
| Resolution | 36.240 - 1.880 |
| R-factor | 0.2078 |
| Rwork | 0.204 |
| R-free | 0.24090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.612 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.19.1_4122) |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.240 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rpim | 0.062 | |
| Number of reflections | 14427 | 850 |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.996 | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM HEPES-KOH (pH 7.5), 200 mM ammonium acetate, and 25% PEG-3350 |
