8SPG
Crystal structure of N-terminally truncated Escherichia coli RapA in complex with MgADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.567, 82.099, 188.546 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.350 - 2.100 |
| R-factor | 0.1987 |
| Rwork | 0.198 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.862 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.125 | 0.040 | 1.603 |
| Rmeas | 0.132 | 0.042 | 1.700 |
| Rpim | 0.044 | 0.014 | 0.562 |
| Total number of observations | 542802 | ||
| Number of reflections | 60199 | 6365 | 5897 |
| <I/σ(I)> | 6.5 | ||
| Completeness [%] | 99.9 | 99.9 | 100 |
| Redundancy | 9 | 8.4 | 9.1 |
| CC(1/2) | 0.998 | 0.999 | 0.498 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG3360 |
