8SOV
Proteinase K Multiconformer Model at 353K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 353 |
| Detector technology | PIXEL |
| Collection date | 2019-06-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03316 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.361, 68.361, 104.043 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.470 - 1.291 |
| R-factor | 0.1318 |
| Rwork | 0.131 |
| R-free | 0.16510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.694 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.470 | 1.337 |
| High resolution limit [Å] | 1.291 | 1.291 |
| Number of reflections | 62442 | 5564 |
| <I/σ(I)> | 13.98 | 0.87 |
| Completeness [%] | 98.8 | 90.59 |
| Redundancy | 7.1 | 6.3 |
| CC(1/2) | 0.999 | 0.333 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Proteinase K was dissolved at pH 7.5 to 30 mg/mL in a 50 mM TRIS buffer. The protein was crystallized using a hanging drop setup on a 24 well VDX plate with sealant and 22 mm thick siliconized circle cover slides by mixing equal amounts of protein solution with 1.2 M ammonium sulfate. |
