8SO8

Crystal structure of an N-terminally truncated variant of Escherichia coli RapA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	122.421, 122.421, 187.795
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.868 - 2.607
R-factor	0.2256
Rwork	0.224
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.566
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.690
High resolution limit [Å]	2.600	5.590	2.600
Rmerge	0.097	0.053	0.651
Rmeas	0.105	0.056	0.772
Rpim	0.036	0.018	0.402
Number of reflections	42303	4487	3820
<I/σ(I)>	10.8
Completeness [%]	95.7	95	88.6
Redundancy	7	9.3	3
CC(1/2)		0.998	0.088

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	Lithium sulfate