8SMJ
Chimeric ETS-domain of murine PU.1 harboring the corresponding beta-strand 3 (S3) residues from murine Ets-1 in complex with d(AATAAGCGGAATGGGG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.92010 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.767, 61.193, 44.774 |
| Unit cell angles | 90.00, 117.28, 90.00 |
Refinement procedure
| Resolution | 24.260 - 1.390 |
| R-factor | 0.1422 |
| Rwork | 0.142 |
| R-free | 0.15400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.140 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.260 | 1.430 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.058 | 0.566 |
| Number of reflections | 41218 | 2998 |
| <I/σ(I)> | 12.3 | 2.1 |
| Completeness [%] | 99.7 | 98 |
| Redundancy | 5.2 | 4.7 |
| CC(1/2) | 0.998 | 0.828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 100 mM Sodium Acetate, pH=4.6, 2% PEG 3350 |
