8SME

Structure of SPO1 phage Tad2 in apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	193
Detector technology	PIXEL
Collection date	2022-12-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 63 2 2
Unit cell lengths	108.402, 108.402, 74.262
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.940 - 2.360
R-factor	0.2664
Rwork	0.263
R-free	0.29870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.420
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.940	2.450
High resolution limit [Å]	2.360	2.360
Rpim	0.019	0.815
Number of reflections	11073	1126
<I/σ(I)>	19.6
Completeness [%]	100.0	100
Redundancy	27	26.7
CC(1/2)	0.999	0.425

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	MES, dioxane, ammonium sulfate