8SLV

Structure of a salivary alpha-glucosidase from the mosquito vector Aedes aegypti.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95.5
Detector technology	PIXEL
Collection date	2021-07-29
Detector	DECTRIS EIGER2 R 4M
Wavelength(s)	1.54178
Spacegroup name	P 1
Unit cell lengths	48.760, 51.030, 70.750
Unit cell angles	101.82, 94.66, 95.76

Refinement procedure

Resolution	22.550 - 2.320
R-factor	0.149
Rwork	0.146
R-free	0.18840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.208
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.790	2.380
High resolution limit [Å]	2.320	2.320
Rmeas	0.039	0.106
Number of reflections	27047	1623
<I/σ(I)>	26.95	11.82
Completeness [%]	94.8	78.4
Redundancy	3.2	2.88
CC(1/2)	0.990	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	0.2 M magnesium chloride hexahydrate, , 0.1 M HEPES sodium pH 7.5, 30% v/v 2-propanol