8SKQ

RNA oligonucleotide containing an alpha-(L)-threofuranosyl nucleic acid (TNA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	32.659, 39.775, 25.728
Unit cell angles	90.00, 107.85, 90.00

Refinement procedure

Resolution	19.890 - 1.350
R-factor	0.2415
Rwork	0.240
R-free	0.27140
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.637
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	HKL2Map
Refinement software	PHENIX (1.19.2-4158-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.890	1.395
High resolution limit [Å]	1.347	1.347
Number of reflections	6922	616
<I/σ(I)>	15.5
Completeness [%]	98.4
Redundancy	19.2
CC(1/2)	0.999	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	1 mM RNA, 1 mM HoCl3, 5 mM spermine 4 HCl, 20 mM sodium cacodylate equilibrated against 40% MPD