8SKI

Crystal structure of human DNA polymerase eta incorporating 5F-dUTP across HX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	120
Detector technology	PIXEL
Collection date	2023-02-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979338
Spacegroup name	P 61
Unit cell lengths	98.560, 98.560, 81.094
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.260 - 2.163
R-factor	0.2021
Rwork	0.200
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o3n
RMSD bond length	0.009
RMSD bond angle	1.105
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.791	2.202
High resolution limit [Å]	2.163	2.163
Number of reflections	24004	1260
<I/σ(I)>	9.665
Completeness [%]	100.0
Redundancy	13.43
CC(1/2)	0.998	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MES, pH 6.5, 20% PEG2000 MME, 5 mM calcium chloride