8SJW

[4T28] Self-assembling right-handed tensegrity triangle with 28 interjunction base pairs and R3 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-24
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	H 3
Unit cell lengths	227.624, 227.624, 91.414
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.470 - 7.640
R-factor	0.1787
Rwork	0.177
R-free	0.19240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w6w
RMSD bond length	0.009
RMSD bond angle	0.893
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	82.931	82.931	8.346
High resolution limit [Å]	7.318	16.578	7.318
Number of reflections	1653	206	209
<I/σ(I)>	7.7	20.1	0.9
Completeness [%]	81.2	99.5
Redundancy	10.9	11	11.1
CC(1/2)	0.999	0.999	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	100 mM MOPS, 1.25 M magnesium sulfate